Research Article
Bartłomiej Grygorcewicz, Magdalena Struk, Agata Wasak, Paweł Nawrotek
West Pomeranian University of Technology, Szczecin, Poland
Abstract. This study was aimed to in vitro evaluation of a bacteriophage isolated from pig slurry samples as a bacteriolytic agent to elimination Shiga toxin–producing E. coli (STEC) O157: H7. Collected STEC O157: H7 strains was susceptible for bacteriophage infection. The phage infection at the multiplicity of infection (MOI) of 1, 5 or 8 caused a rapid cell lysis resulting in lack of growth in the cell concentration. In conclusion, the data obtained from the present study shows that bacteriophage isolated from pig slurry is a lytic bacteriophage capable to killing strains of E. coli O157: H7. Our results demonstrate that specific bacteriophages have the potential to biocontrol STEC strains in environment.
Keywords: Key words: STEC, E. coli O157: H7, bacteriophages, biocontrol, pig slurry
Accepted: 24 Mar 2015
Published online: 30 Apr 2017
Accesses: 406
Grygorcewicz, B., Struk, M., Wasak, A., Nawrotek, P., (2015). Effectiveness bacteriolysis of Shiga toxin-producing Escherichia coli O157: H7 caused by specific bacteriophage isolated from pig slurry. Acta Sci. Pol. Zootechnica, 14(1), 69–76.